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Southern Federal University

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Marina Alexandrovna Sazykina 

Professor

Academy of Biology and Biotechnologies

Senior researcher

Academy of Biology and Biotechnologies

E-mail:
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Degree: Doctor of Sciences

Personal page in Russian:
https://sfedu.ru/person/samara
Personal page in English:
https://sfedu.ru/en/person/samara

Research interests:

Under the guidance of M.A. Sazykina research on biochemistry of oil-degrading bacteria and monitoring the environment using bacterial sensors is conducted in the Academy of Biology and Biotechnology of SFedU named after D.I. Ivanovsky. The collection of oil-degrading microorganisms isolated from the Kerch Strait has been created. The possibility of non-enzymatic oxidation of various oil components with the free-radical mechanism has been shown [1-3].

The scientific concept of using biosensors based on bioluminescent bacteria in toxicity monitoring of aquatic ecosystems enhancing the efficiency of monitoring has been created. The test system for monitoring of ecosystems contamination, based on the use of the complex of luminescent biosensors has been designed and implemented. The draft of methodological recommendations named "Assessment of the ecosystems components toxicity using a battery of bacterial lux-biosensors" has been produced [4].

The test system for assessing the toxicity of the components of marine waters ecosystems on the basis of native microalgae fluorimetry has been developed [5-6].

The new species of bioluminescent bacteria of the Vibrio genus promising for toxicity testing has been isolated from the Black Sea water. The name of Vibrio aquamarinus sp. nov. was given to the strain. The strain has been deposited in the Russian National Collection of Industrial Microorganisms (Vibrio aquamarinus VKPM B-11245) and in the German Collection of Microorganisms and Cell Cultures (Vibrio aquamarinus DSM 26054) [4].

Three strains of bioluminescent bacteria isolated from the water of the Black sea have been proposed as new test cultures for environment components toxicity testing [4, 7].

Lux-operons of these microorganisms can be used for further construction of the bacterial luminescent sensors.

For the first time the results on toxicity, genotoxicity, prooxidant activity, presence of mercury and arsenic, and substances damaging proteins and membranes in water of 23 springs of Rostov-on-Don in the period from 2009 to 2012 have been obtained. Polyaromatic hydrocarbons participation in the development of prooxidant effect and genotoxicity of spring water in Rostov- on-Don has been revealed [8-9].

The results of bottom sediments monitoring of the Lower Don during the years 2003-2015 allowed to register the time periods with the maximum pollution with genotoxic substances [10]1 The chronic character of pollution with genotoxicants of a number of the studied areas has been discovered. It is revealed that the source of bottom sediments genotoxicity of the Lower Don are oil products, individual PAHs (phenanthrene, fluoranthene, pyrene, chrysene, benzo(k)fluoranthene, benzo(a)pyrene), vanadium, nickel and mercury [11, 12].

According to the studies of the Azov and the Black Sea basin ecosystems exposed to anthropogenic pressure in 2014-2016 the nomenclature of clinically relevant resistance determinants characteristic for the studied biotopes was compiled.

Databases on ecosystems contamination in the Rostov region have been created [14-16].

A number of culture media for cultivation of Deinococcus radiodurans [17, 18] has been developed.

Chromatographic method of separation of Deinococcus radiodurans carotenoids using low pressure adsorption chromatography to obtain deinoxanthin with unique high antioxidant activity has been developed [19].

The technology of synbiotic preparations based on biofilms of Bacillus genus bacteria immobilized on plant substrates has been created.

The method for assessing the effectiveness of antibiotics and antimicrobial effects of the ultrasound radiation on pathogenic bacteria existing in the form of a biofilm was proposed and patented [20].

For a number of biotechnological developments M.A. Sazykina was awarded with the Diploma of the Government of the Rostov region of I degree "The best inventor and innovator of the Rostov Region"

(2013), and the staff of the laboratory under her leadership was awarded with the small and the big gold medals of the 6th International Biotechnology Fair "RosBioTech 2012" for the development probiotic preparations production technology based on Bacillus subtilis, and the technology of bioremediation of oil contaminated soils.

Currently, the laboratory headed by M.A. Sazykina conducts joint research of: biological activity of newly synthesized drugs with scientists from the A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences; ecotoxicology of aquatic ecosystems with scientists from the Institute of Hygiene and Medical Microbiology of Max von Pettenkofer Ludwig Maximilian University of Munich (Germany).

The data obtained with the help of bacterial lux-biosensors showed that the integral toxicity of wastewaters in Munich and Rostov-on-Don during the observation period was similar. But a greater range of antibiotic resistance genes was detected in the wastewaters of Rostov-on-Don [21-24].

The UV protective activity of lipophilic cations, which contain phenolic or sterically hindered phenolic moieties [25-27], as well as UV-protective and antibacterial activity of novel hybrid benzofuroxane compounds and their derivatives [28-33] have been revealed.

Acting as a manager or an executive M.A. Sazykina took part in implementation of 27 projects. She is a member of the "Ecology of soil" leading scientific school of the Russian Federation. She is a reviewer of the journal «Folia Microbiologica», «Soil and Sediment Contamination. An International Journal », «International Journal of Environmental Science and Technology», «Living and biokosnye systems", “University news. North-caucasian region. Natural sciences series ".

She is the author of  123 publications (in the last 5 years), including 8 monographs, 18 databases, 5 invention patents and 33 articles in peer-reviewed journals.

 

References

  1. Sazykin I.S., Sazykina M.A. 2013. Effect of antioxidants on microbial transformation of oil // Water: chemistry and ecology. - N 3. - S. 75-80.
  2. Sazykin I.S., Sazykina M.A. Suholentseva J.V., Hammami M.I. generation of superoxide-anion by oil oxidizing microorganisms Acinetobacter calcoaceticus during incubation with various hydrocarbons // Water: chemistry and ecology. – 2014. - N 9. – P. 113-117.
  3. Sazykin I.S., Sazykina M.A., Khmelevtsova L.E., Khammami M.I., Karchava Sh.K., Zhuravlevа M.V., Kudeevskaya E.M. Expression of SOD and Production of Reactive Oxygen Species in Acinetobacter calcoaceticus Caused by Hydrocarbons Oxidation // Annals of microbiology. – 2016. – V.  66. -  N 3. – P. 1039–1045. DOI 10.1007/s13213-015-1188-9
  4. Sazykina M.A. Ecotoxicological assessment of aquatic ecosystems by using biosensors based on luminescent bacteria: Thesis. Dis. ... Dr. biol. Sciences. Rostov-on-Don. 2014.
  5. Afanasiev D.F., Tsybulsky I.E., Larin A.A., Korpakova I.G., Sazykina M.A., Morozova M.A., Volovik S.P. 2012. Development of test systems for assessment of the components toxicity of marine ecosystems by fluorimetry of native microalgae // News of higher educational institutions. North Caucasus region. Science. - N 4. - P. 55-59.
  6. Pat. No. 2013 131 303. S12N 1/12, C02F 9/04, C12Q 1/06. A method for producing bacteriological pure cultures of marine microalgae / Afanasiev D.F., Sazykina M.A., Tsybulsky I.E., Korpakova I.G., Morozova M.A.; N: 2013131303/10; 07/08/2013; publ. 01.20.2015, Bull. N 2.
  7. Pat. 2534 819. The Russian Federation. IPC S12N1 / 20 S12R1 / 63, C12Q1 / 02. The strain of Vibrio aquamarinus. A method for determining the toxicity of samples with it and test culture for toxicity determination of samples / Sazykin I.S., Sazykina M.A., Kudeevskaya E.M., Sazykina M.I. - 2012148361/10, stating . 13.11.2012; publ. 10.12.2014, Bull. N 34- p.9: Fig. , Table. 4.
  8. Sazykina M.A., Khatab Z.S., Kudeevskaya E.M., Sazykin I.S. 2013. Assessment of the quality of water springs in Rostov-on-Don on the basis of microbiological and toxicological indicators // Water: chemistry and ecology. - N 1. - P. 102-107.
  9. The software package "Database of the water springs toxicity of Rostov-on-Don", a registration certificate of an electronic resource number 20531 / Hammami M.I., Kostina N.V., Hmelevtsova L..E, Varduni T.W., Sazykin I.S., Sazykina M.A. Number 50201450787; appl. 03/09/2014; issued 11.20.2014.
  10. Sazykina M.A., Chistyakov V.A., Sazykin I.S. 2012. Genotoxicity of Don River bottom sediments (2001-2007) // Water Resources. - V. 39. - N. - P. 118-124.
  11. The software package "Database of the sediments toxicity of the Lower Don", a registration certificate of an electronic resource number 20532 / Kudeevskaya E.M., Kostina N.V., Hammami M.I., Hmelevtsova L.E., Sazykin I. S., Sazykina M.A. Number 50201450788; appl. 03/09/2014; issued 11.20.2014.
  12. Sazykin I.S., Sazykina M.A., Khammami M.I., Khmelevtsova L.E., Kostina N.V., Trubnik R.G. 2015. Distribution of polycyclic aromatic hydrocarbons in surface sediments of lower reaches of the Don River (Russia) and their ecotoxicologic assessment by bacterial lux-biosensors // Environ. Monit. Assess. - V. 187 (5): 4406. DOI: 10.1007 / s10661-015-4406-9
  13. Sazykina M.A., Sazykin I.S., Khammami M.I., Kudeevskaya E.M., Seliverstova E. Y. Biosensor analysis of anthropogenic pollution of the Lower Don bottom sediments // Journal " Bulletin of biotechnology and physical and chemical biology named after Y.A. Ovchinnikov "- 2016 - V.12. - N 1. - P. 5-11.
  14. The software package  «Database on the antibiotics content in Rostov-on-Don wastewaters», a registration certificate of an electronic resource number 21258 / Zhuravleva M.V., Karchava Sh.K., Kudeevskaya E.M., Sazykin I.S., Sazykina M.A. N 50201550485; appl.  15.10.2015;   issued 27.10.2015.
  15. The software package «Database on the antibiotics content in water ecosystems of the Rostov Region»: a registration certificate of an electronic resource number 22216 /  Zhuravleva M.V., Sazykin I.S., Sazykina M.A., Karchava Sh.K., Hmelevtsova L.E., Kudeevskaya E.M., M.I. Hammami, Seliverstova E.Y. N 50201650442; appl.  20.10.2016;   issued 20.10.2016.
  16. The software package «Database on the antibiotics content in the soils of the Rostov region exposed to anthropogenic pollution» a registration certificate of an electronic resource number 22215 / Karchava Sh.K., Sazykina M.A., Sazykin I.S., Zhuravleva M.V., Kudeevskaya E.M., Hmelevtsova L.E., Hammami M.I., Seliverstova E.Y. N 50201650443; appl.  20.10.2016;   issued 20.10.2016.
  17. Pat. 2418061. The Russian Federation, the IPC C12N1 / 10, C12R1 / 00. The nutrient medium for the cultivation of microorganisms Deinococcus radiodurans / Sazykina M.A., Chistyakov V.A., Sazykin I.S., Sazykina M.I.; 2009114061/10, stated. 2009.04.13 .; publ. 2011.05.10, Bul. N 13.- .: 9 Table 2.
  18. Pat. 2013134369. "The medium for the cultivation of microorganisms Deinococcus radiodurans», application N 2013134369/10 (051395). Chistyakov V.A., Sazykina M.A., Sazykin I.S., Prazdnova E.V.; Application N 2013134369/10, appl. 07/22/2013; publ. 01.27.2015, Bull. Number 3 22.
  19. Pat. 2475541. The Russian Federation, the IPC S12R23 / 00 S12N1 / 20, C07C403 / 00, C12R1 / 01. A method for deinoksantin production - carotenoid of microorganism Deinococcus radiodurans / Sazykin I.S., Sazykina M.A., Chistyakov V.A.; N 2011139309/10, appl. 26.09.2011; publ. 20.02.2013, Bull. Number 5 - 9 p .: fig. 2, Table. 1.
  20. Pat. 2457254. The Russian Federation. IPC C12Q 1/04 , C12N 13/00. Method for estimating effectiveness of antimicrobial action of antibiotics and ultrasound on pathogenic bacteria in form of biofilm / Sazykin I.S., Sazykina M.A., Chistjakov V.A., Lysenko V.S. - 2011106708/10, stated. 22.02.11; publ. 27.07.2012, Bull. N 21- p.26: fig.25.
  21. Sazykina M.A., Sazykin I.S., Kostina N.V., Hmelevtsova L.E., Trubnik R.G., Sazykina M.I. 2014. Research ecotoxicological parameters of Rostov-on-Don and Munich wastewater // Water: chemistry and ecology. - N 1. - P. 3-10.
  22. The software package «Database on the toxicity of Rostov-on-Don wastewaters», a registration certificate of an electronic resource number 21257 /  Mirina E.A., Hmelevtsova L.E., Hammami M.I., Sazykin I.S., Sazykina M.A. N 50201550490; appl.  15.10.2015;   issued 27.10.2015.
  23. The software package «Database on the toxicity of Munich wastewaters», a registration certificate of an electronic resource number 21256 /  Karchava Sh.K., Zhuravleva M.V., Hammami M.I., Sazykin I.S., Sazykina M.A. N 50201550485; appl.  15.10.2015;   issued 27.10.2015.
  24. Sazykin I.S., Sazykina M.A.,  Khmelevtsova L.E., Mirina E.A, Kudeevskaya E.M., Rogulin E.A., Rakin A.V. Biosensor-based comparison of the ecotoxicological contamination of the wastewaters of Southern Russia and Sourthern Germany // International Journal of Environmental Science and Technology. - 2016. – V. 13. - N3. -  P. 945-954. - DOI 10.1007/s13762-016-0936-0
  25. Oludina Yu.N., Sazykina M.A., Prazdnova E.V., Sazykin I.S., Khmelevtsova L.E., Bukharov S.V., Barsukova T.A., Tagasheva R.G., Nugumanova G.N. // Synthesis of Modified Sterically Hindered Phenols and Investigation of Their Ability to Protect Bacterial DNA Against UVB Damage // Pharmaceutical Chemistry Journal. 2015; V. 48(12):787-790. DOI:10.1007/s11094-015-1196-x
  26. Tagasheva R.G., Bukharov S.V., Nugumanova  G. N., Oludina Yu.N.,  Sazykina M.A., I. S. Sazykin, E.Yu. Seliverstov, Khammami M.I.. Synthesis and antioxidant activity of modified sterically hindered phenols  // Russian Journal of General Chemistry. – V.86. - N 3. - P. 602-606.  DOI: 10.1134/S1070363216030166 
  27. The software package «Database on biological activity of modified sterically hindered phenols »: a registration certificate of an electronic resource number 22221 / Sazykina M.A., Bukharov S.V., Sazykin I.S., Tagasheva R.G., Karchava Sh.K., Oludina Y.N., Zhuravleva M.V., Nugumanova G.N., Hmelevtsova L.E., Barsukova T.A., Hammami M.I., Kudeevskaya E.M., Seliverstova E.Y. N 50201650439; appl.  20.10.2016;  issued 20.10.2016.
  28. The software package « Database on the biological activity of benzofuroxane derivatives»: a registration certificate of an electronic resource number 22222/  Sazykina M.A., Chugunova E.A., Sazykin I.S, Burilov A.R., Karchava Sh.K., Muhamatdinova R.E., Zhuravleva M.V., Hmelevtsova L.E., Hammami M.I., Kudeevskaya E.M., Gavrilov N.V., Seliverstova E.Y. N 50201650438; appl.  20.10.2016;   issued 20.10.2016.
  29. Chugunova, E.A., Sazykina, M.A.,Gibadullina, E.M., Burilov, A.R., Sazykin, I.S., Chistyakov V.A., Timasheva R.E., Krivolapov D.B, Goumont R. Synthesis, Genotoxicity and UV-protective activity of new benzofuroxans substituted by aromatic amines // Letters in Drug Design & Discovery. - 2013. - V. 10. - N 2. - pp. 145-154.
  30. Chugunova E., Boga C., Sazykin I., Cino S., Micheletti G., Mazzanti A., Sazykina M., Burilov A., Khmelevtsova L., Kostina N. Synthesis and antimicrobial activity of novel structural hybrids of benzofuroxan and benzothiazole derivatives // Eur. J. Med. Chem. – 2015. – V. 93. – P. 349–359. doi:10.1016/j.ejmech.2015.02.023
  31. Chugunova E.A., Mukhamatdinova R., Sazykina M., Dobrynin A., Sazykin I., Karpenko A., Mirina E., Zhuravlevа M., Karchava S., Burilov A. Synthesis of new "Hybrid" Compounds based on Benzofuroxans and Aminoalkylnaphthalimides Hybrid" Compounds based on Benzofuroxans // Chemical Biology & Drug Design. – 2016. – V. 87 - N 4. - P. 626-634. DOI 10.1111/cbdd.12685
  32. Chugunova E.A., Mukhamatdinova R.E., Sazykina M.A., Sazykin I.S., Gavrilov N.V., Dobrynin A.B., Kudeevskaya E.M., Burilov A.R. Novel hybrid compounds derived from benzofuroxans and sulfonamides // Russian Journal of General Chemistry. – 2016. – V. 86(5). - P. 1032–1036.  DOI: 10.1134/S107036321605008X 
  33. Chugunova E.A., Mukhamatdinova R.E., Sazykina M.A., Sazykin I.S., Khammami M. I., Akylbekov N.I., Burilov A.R., Kulik N.V., Zobov  V.V. Synthesis and biological activity of new hybrids compounds derived from benzofuroxanes and polyene antibiotics // Russian Journal of General Chemistry. - 2016. - 86(5):1037-1040.  DOI: 10.1134/S1070363216050091

 

 

Research projects:

Leader:
1. Analytical departmental target program "Development of the scientific potential of the higher school (2009-2011)", project No. 2.1.1 / 5028 Monitoring of environmental pollution with bacterial lux-biosensors ", 2009-2010.
2. Analytical departmental target program "Development of the scientific potential of higher education (2009-2011)", project No. 2.1.1 / 5232 "Study of free-radical mechanisms of biodegradation of hydrocarbons", 2009-2010.
3. Research performed on the instructions of the Ministry of Education and Science of the Russian Federation (registration number 01201178792) on the topic: "Investigation of air pollution and atmospheric precipitation of urbanized areas using the example of Rostov-on-Don using bacterial lux-biosensors", 2011.
4. Research performed on the instructions of the Ministry of Education and Science of the Russian Federation (registration number 01201178793) "Investigation of the role of free radical processes in the phenomenon of comet metabolism of oil components by microorganisms, 2011.
5. Research performed on the instructions of the Ministry of Education and Science of the Russian Federation on the topic: "Investigation of the mechanisms of action of negative anthropogenic and extreme environmental factors with the help of cellular biosensors", 2012-2013.
6. Research performed on the instructions of the Ministry of Education and Science of the Russian Federation on the theme "Development of the Fundamentals of Biotechnology Based on Extremo-Tolerant Microorganisms", 2012-2013.
7. Federal target program "Scientific and scientific-pedagogical staff of innovative Russia" for 2009-2013, activity 1.5, project No. 14.F18.21.0851 "Study of ecotoxicological parameters and bacterial pollution of sewage in Rostov-on-Don and Munich, 2012-2013. (Head of the Russian side).
8. Grant of the Southern Federal University No. 213.01-07-2014 / 12HPHVG "Monitoring and studying the influence of pollutants provoking the emergence and transmission of bacterial determinants of resistance in biotopes of the Azovo-Black Sea basin subject to anthropogenic pressure", 2014-2016.

Executor:
1. Analytical departmental target program "Development of the scientific potential of the higher school (2009-2011)", project No. 2.1.1 / 5628 "Investigation of the biological effects of ultraviolet radiation of wavelength 300-400 nm with bacterial lux-biosensors", 2009-2010 Years. (01.01.2009 - 31.12.2010).
2. Analytical departmental target program "Development of the scientific potential of higher education (2009-2011)", project No. 2.1.1 / 5630 "Investigation of antioxidant properties of bacterial origin of carotenoids", 2009-2010. (01.01.2009 - 31.12.2010)
3. The federal special-purpose program "Research and development in priority areas of development of the scientific and technological complex of Russia for 2007-2013," GC No. 02.552.11.7072 "Conducting research and research in the field of micro- and nanostructures based on oxide, organic and biological Materials, the development of technology for their production for the development of a promising sensor system based on new physical principles in the center of collective use of scientific equipment "High Technologies", 2009-2010. (02.10.2009 - 30.10.2010).
4. The project "Practical use of Skulachev ions" (2010).
5. Research (registration number 01201178794), conducted by the Ministry of Education and Science of the Russian Federation "Investigation of the UV-protective activity of lipophilic cations containing phenolic or hindered phenolic fragments", 2011 (01.01.2011 - 31.12.2011 ).

6. Research work (registration number No. 01201178795, commissioned by the Ministry of Education and Science of the Russian Federation "Study of the regenerative activity of the carotenoid fraction of Deinococcus radiodurans in the model of a full-thickness cutaneous skin on the background of type I diabetes in mice", 2011 (01.01.2011 - 31.12.2011 ).
7. Federal target program "Research and development in priority areas for the development of the scientific and technological complex of Russia for 2007-2013," GK No. 16.512.11.2017 "Development of technology for synbiotic drugs based on immobilized on vegetable substrate biofilms of bacteria of the genus Bacillus", 2011-2012 Years. (17.02.2011-30.11.2012)
8. Basic financing, project No. 1-K-11-30 / 1 "Development of methods for the preparative separation of carotenoids of Deinococcus radiodurans", 0.3 million rubles, 2011 (01.06.2011-30.12.2011).
9. Foundation for Assistance to Small Innovative Enterprises in Science and Technology, Project No. 9397p / 15144 "Development of a technological minicomplex for the production of probiotic preparations based on Bacillus subtilis and Lactobacillus plantarum in conditions of personal subsidiary and small livestock and poultry farms", 1 , 0 million rubles., 2011-2012. (30/05/2011-30/05/2012).
10. Basic financing, project No. 01201366519 "Study of the generation of oxygen radicals by oil-oxidizing microorganisms in the biotransformation of hydrocarbons," 0.3 million rubles, 2013 (beginning 01.05.2013, ending December 15, 2013).
11. Basic funding, project No. 01201366515 "Investigation of mechanisms of antioxidant activity of fullerene C60", 2013 (beginning of 01/05/2013, ending on December 15, 2013).
12. Grant of the President of the Russian Federation for state support of the leading scientific schools of the Russian Federation No. NSh-2449.2014.4 "Ecological functions of soils under anthropogenic impact" of 03.02.2014, 2014 ((01.01.2014 - 31.12.2015).
13. Project No. 1894, carried out within the basic part of the state task of the Ministry of Education and Science of the Russian Federation "Biomonitoring of the state of ecosystems in the Azovo-Black Sea basin", 2014 (01.01.2014 - 31.12.2016).
14. Project No. 213.01-2014 / 007 "Integrated Ecological-Toxicological Assessment of Water Resources in the Azov-Black Sea Basin", implemented within the framework of the internal part of the SFU internal grant, 2014.
19. Grant of the President of the Russian Federation for state support to the leading scientific schools of the Russian Federation No. NSh-9072.2016.11 "Ecological and agricultural functions of soils and in conditions of anthropogenic load", 2016-2017.

 

Teaching:

  • Virology
  • Microbial transformations. Antibiotics and their producers
  • Microbiology and basis of biotechnology